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Correlation between ebv co-infection and HPV16 genome integrity in Tunisian cervical cancer patients BJM
Kahla,Saloua; Oueslati,Sarra; Achour,Mongia; Kochbati,Lotfi; Chanoufi,Mohamed Badis; Maalej,Mongi; Oueslati,Ridha.
Infection with high risk Human papillomavirus (HR-HPV) is necessary but not sufficient to cause cervical carcinoma. This study explored whether multiple HR-HPV or coinfection with Epstein-Barr virus (EBV) influence the integration status of HPV16 genome. The presence and typing of HPV in a series of 125 cervical specimens were assessed by polymerase chain reaction (PCR) using the specific primers for the HPV L1 region. As for EBV infection, the viral EBNA1 gene was used for its detection through PCR amplification. Disruption of the HPV E2 gene was assessed by amplification of the entire E2 gene with single set of primers, while E2 transcripts were evaluated by a reverse transcription PCR method (RT-PCR). The overall prevalence of HPVDNA was of 81.8% in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cervical cancer; Epstein-Barr virus; E2 gene; E2 mRNA; High risk human papillomaviruses.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200039
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